Ro15-4513

• CAT Number: I009091
• CAS Number: 91917-65-6
• Molecular Formula: C15H14N6O3
• Molecular Weight: 326.31
• Purity: ≥95%
• Synonyms: ethyl 8-azido-5-methyl-6-oxo-4H-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate
• InChI: InChI=1S/C15H14N6O3/c1-3-24-15(23)13-12-7-20(2)14(22)10-6-9(18-19-16)4-5-11(10)21(12)8-17-13/h4-6,8H,3,7H2,1-2H3
• InChIKey: CFSOJZTUTOQNIA-UHFFFAOYSA-N
• SMILES: CCOC(=O)C1=C2CN(C(=O)C3=C(N2C=N1)C=CC(=C3)N=[N+]=[N-])C

Ro15-4513(CAT: I009091) is an imidazobenzodiazepinone derivative acting primarily as a partial inverse agonist at benzodiazepine receptors, with agonist activity at GABAA receptors containing α4 and α6 subunits. Notably, Ro15-4513 is a potent ethanol antagonist, capable of reversing ethanol-induced sedation and locomotor suppression in vivo. It also exhibits anxiolytic properties in preclinical models. Beyond pharmacology, Ro15-4513 contains an azide functional group, enabling bioorthogonal conjugation via copper-catalyzed azide–alkyne cycloaddition (CuAAC) and strain-promoted azide–alkyne cycloaddition (SPAAC). This dual functionality makes Ro15-4513 a versatile tool for studying GABAergic signaling, ethanol antagonism, and chemical biology applications using click chemistry.