RETF-4NA TFA

• CAT Number: I045797
• Molecular Formula: C34H44F3N9O12
• Molecular Weight: 827.76
• Purity: ≥95%
• IUPAC Name: (4S)-4-[[(2S)-2-acetamido-5-(diaminomethylideneamino)pentanoyl]amino]-5-[[(2S,3R)-3-hydroxy-1-[[(2S)-1-(4-nitroanilino)-1-oxo-3-phenylpropan-2-yl]amino]-1-oxobutan-2-yl]amino]-5-oxopentanoic acid;2,2,2-trifluoroacetic acid
• InChI: InChI=1S/C32H43N9O10.C2HF3O2/c1-18(42)27(40-29(47)24(14-15-26(44)45)38-28(46)23(36-19(2)43)9-6-16-35-32(33)34)31(49)39-25(17-20-7-4-3-5-8-20)30(48)37-21-10-12-22(13-11-21)41(50)51;3-2(4,5)1(6)7/h3-5,7-8,10-13,18,23-25,27,42H,6,9,14-17H2,1-2H3,(H,36,43)(H,37,48)(H,38,46)(H,39,49)(H,40,47)(H,44,45)(H4,33,34,35);(H,6,7)/t18-,23+,24+,25+,27+;/m1./s1
• InChIKey: ZCKAWGNYQYWPQO-RTNJFBSRSA-N
• SMILES: C[C@H]([C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)C)O.C(=O)(C(F)(F)F)O

RETF-4NA TFA（Cat No.:I045797）is a synthetic fluorogenic peptide substrate designed for protease activity assays. It contains the RETF sequence linked to 4-nitroanilide (4NA), which is released upon enzymatic cleavage, producing a measurable chromogenic or fluorogenic signal. This property enables sensitive, quantitative monitoring of specific protease activity in biochemical and cellular systems. Supplied as a trifluoroacetate (TFA) salt, it offers enhanced solubility and stability for experimental applications. RETF-4NA TFA is widely used in enzymology, drug discovery, and protease research to study catalytic mechanisms, inhibitor screening, and pathway regulation.