Propidium Iodide

• CAT Number: R001023
• CAS Number: 25535-16-4
• Molecular Formula: C27H34N4 • 2I
• Molecular Weight: 668.40
• Purity: ≥95%
• Synonyms: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium Iodide; 3,8-Diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridinium Iodide Methiodide; [3-(3,8-Diamino-6-phenyl-5-phenanthridinio)propyl]diethylmethylammonium Diiodide; 3,8-D
• Target: Reagents
• Solubility: Soluble to 5 mM in sterile water with sonication
• Storage: Store at -20C
• IUPAC Name: 3-(3,8-diamino-6-phenylphenanthridin-5-ium-5-yl)propyl-diethyl-methylazanium;diiodide
• InChI: InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
• InChIKey: XJMOSONTPMZWPB-UHFFFAOYSA-M
• SMILES: CC[N+](C)(CC)CCC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[I-].[I-]

Propidium Iodide (Cat No.: R001023) is a fluorescent, DNA-binding dye commonly used to assess cell viability and apoptosis. It intercalates into double-stranded DNA but is impermeable to live cells, making it ideal for distinguishing dead or membrane-compromised cells in flow cytometry or microscopy. PI emits red fluorescence upon DNA binding, typically used alongside other markers like Annexin V in apoptosis assays. Due to its inability to penetrate intact membranes, PI is a reliable indicator of late apoptosis or necrosis.