N-Hydroxysuccinimide

• CAT Number: R006081
• CAS Number: 6066-82-6
• Molecular Formula: C4H5NO3
• Molecular Weight: 115.09
• Purity: ≥95%
• Synonyms: 1-Hydroxy-2,5-pyrrolidinedione; N-Hydroxy-2,5-dioxopyrrolidine; 1-Hydroxysuccinimide; NSC 74335; NHS
• Storage: Room temperature
• IUPAC Name: 1-hydroxypyrrolidine-2,5-dione
• InChI: InChI=1S/C4H5NO3/c6-3-1-2-4(7)5(3)8/h8H,1-2H2
• InChIKey: NQTADLQHYWFPDB-UHFFFAOYSA-N
• SMILES: C1CC(=O)N(C1=O)O

N-Hydroxysuccinimide (Cat No.:R006081) is a cyclic organic compound used widely in biochemistry and organic synthesis, particularly for its role in the activation of carboxyl groups in peptide synthesis and coupling reactions. NHS forms stable ester linkages with amines, facilitating the formation of amide bonds in the presence of carbodiimide reagents such as EDC. It is commonly used in the preparation of bioconjugates, such as antibodies or proteins, for drug delivery, diagnostic assays, and molecular biology applications. NHS is typically supplied as a white crystalline powder, soluble in water and organic solvents.