N-CBZ-Phe-Arg-AMC TFA

• CAT Number: I043797
• Molecular Formula: C35H37F3N6O8
• Molecular Weight: 726.70
• Purity: ≥95%
• Synonyms: benzyl N-[(2S)-1-[[(2S)-5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]carbamate;2,2,2-trifluoroacetic acid
• InChI: InChI=1S/C33H36N6O6.C2HF3O2/c1-21-17-29(40)45-28-19-24(14-15-25(21)28)37-30(41)26(13-8-16-36-32(34)35)38-31(42)27(18-22-9-4-2-5-10-22)39-33(43)44-20-23-11-6-3-7-12-23;3-2(4,5)1(6)7/h2-7,9-12,14-15,17,19,26-27H,8,13,16,18,20H2,1H3,(H,37,41)(H,38,42)(H,39,43)(H4,34,35,36);(H,6,7)/t26-,27-;/m0./s1
• InChIKey: AVPSADNFAQBJBZ-WMXJXTQLSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC3=CC=CC=C3)NC(=O)OCC4=CC=CC=C4.C(=O)(C(F)(F)F)O

N-CBZ-Phe-Arg-AMC TFA（Cat No.:I043797）is a peptide substrate used in enzymatic assays to measure the activity of proteases, particularly those involved in cleavage at arginine (Arg)-containing sites. The compound consists of a phenylalanine (Phe) and arginine (Arg) sequence, with a 7-amino-4-methylcoumarin (AMC) fluorogenic group attached. Upon protease cleavage, the AMC group is released, producing fluorescence that can be quantified. N-CBZ-Phe-Arg-AMC TFA is commonly used in research to study protease activity, enzyme kinetics, and to screen for potential inhibitors of enzymes such as trypsin-like proteases, making it a valuable tool in biochemical studies.