N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide

• CAT Number: I040417
• CAS Number: 216757-29-8
• Molecular Formula: C28H38N6O11
• Molecular Weight: 634.63
• Purity: ≥95%
• Synonyms: (4S)-4-[[(2S,3S)-2-acetamido-3-methylpentanoyl]amino]-5-[(2S)-2-[[(2S)-3-carboxy-1-(4-nitroanilino)-1-oxopropan-2-yl]carbamoyl]pyrrolidin-1-yl]-5-oxopentanoic acid
• InChI: InChI=1S/C28H38N6O11/c1-4-15(2)24(29-16(3)35)27(42)31-19(11-12-22(36)37)28(43)33-13-5-6-21(33)26(41)32-20(14-23(38)39)25(40)30-17-7-9-18(10-8-17)34(44)45/h7-10,15,19-21,24H,4-6,11-14H2,1-3H3,(H,29,35)(H,30,40)(H,31,42)(H,32,41)(H,36,37)(H,38,39)/t15-,19-,20-,21-,24-/m0/s1
• InChIKey: YXEIIOVAGGAMCZ-NYMCBPKFSA-N
• SMILES: CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)C

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (CAT: I040417) is a colorimetric substrate specifically designed for the detection and quantification of granzyme B activity. Featuring the peptide sequence IEPD, which is selectively recognized and cleaved by granzyme B, the substrate releases p-nitroaniline (pNA) upon enzymatic hydrolysis. The liberated pNA produces a measurable yellow color, allowing precise monitoring of enzyme kinetics through absorbance at 405 nm. Ac-IEPD-pNA is widely used in immunology and cytotoxicity research to assess granzyme B function in natural killer (NK) and cytotoxic T lymphocyte (CTL) assays. It provides a reliable tool for evaluating immune cell-mediated apoptosis and screening granzyme B modulators.