ML-180

• CAT Number: R065005
• CAS Number: 863588-32-3
• Molecular Formula: C20H25ClN4O2
• Molecular Weight: 388.89
• Purity: ≥95%
• Synonyms: 6-[4-(3-chlorophenyl)piperazin-1-yl]-3-cyclohexyl-1H-pyrimidine-2,4-dione
• InChI: InChI=1S/C20H25ClN4O2/c21-15-5-4-8-17(13-15)23-9-11-24(12-10-23)18-14-19(26)25(20(27)22-18)16-6-2-1-3-7-16/h4-5,8,13-14,16H,1-3,6-7,9-12H2,(H,22,27)
• InChIKey: CHMQQIFPOIZPIJ-UHFFFAOYSA-N
• SMILES: C1CCC(CC1)N2C(=O)C=C(NC2=O)N3CCN(CC3)C4=CC(=CC=C4)Cl

ML-180 (CAT: R065005) is a potent inverse agonist of the orphan nuclear receptor liver receptor homolog 1 (LRH-1; NR5A2) with an IC₅₀ of 3.7 µM, showing inactivity toward steroidogenic factor-1 (SF-1; NR5A1; IC₅₀ >10 µM). In hepatic cells, ML-180 inhibits LRH-1 signaling, suppresses cyclin-D1/E1 expression, and reduces LRH-1 mRNA and downstream targets (CYP19, GATA3, GATA4) in a dose-dependent manner. It efficiently blocks proliferation in LRH-1-positive Huh-7 cells (EC₅₀ ≈ 2.8 µM). In vivo, ML-180 (30 mg/kg, i.p.) decreases LRH-1 and SHP mRNA in adrenal and pancreatic tissues, supporting its potential in LRH-1-dependent cancer research.