Methyltetrazine-PEG5-methyltetrazine

• CAT Number: I045952
• Molecular Formula: C34H44N10O7
• Molecular Weight: 704.78
• Purity: ≥95%
• Synonyms: N-[[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]methyl]-3-[2-[2-[2-[2-[3-[[4-(6-methyl-1,2,4,5-tetrazin-3-yl)phenyl]methylamino]-3-oxopropoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanamide
• InChI: InChI=1S/C34H44N10O7/c1-25-37-41-33(42-38-25)29-7-3-27(4-8-29)23-35-31(45)11-13-47-15-17-49-19-21-51-22-20-50-18-16-48-14-12-32(46)36-24-28-5-9-30(10-6-28)34-43-39-26(2)40-44-34/h3-10H,11-24H2,1-2H3,(H,35,45)(H,36,46)
• InChIKey: WDEMZBSBIMOGBN-UHFFFAOYSA-N
• SMILES: CC1=NN=C(N=N1)C2=CC=C(C=C2)CNC(=O)CCOCCOCCOCCOCCOCCC(=O)NCC3=CC=C(C=C3)C4=NN=C(N=N4)C

Methyltetrazine-PEG5-methyltetrazine(CAT: I045952) is a symmetric homobifunctional linker containing two methyltetrazine groups connected by a five-unit polyethylene glycol (PEG5) spacer. The methyltetrazine moieties enable rapid, catalyst-free bioorthogonal conjugation through inverse electron-demand Diels–Alder (IEDDA) cycloaddition with strained trans-cyclooctenes (TCO). This dual-reactive design allows for efficient crosslinking, dimerization, or multivalent assembly of biomolecules. The PEG5 spacer enhances aqueous solubility, reduces steric hindrance, and provides optimal flexibility for conjugation in biological environments. Methyltetrazine-PEG5-methyltetrazine is a versatile tool in chemical biology, enabling the construction of antibody–drug conjugates, inhibitor scaffolds, and imaging probes for cancer, immunology, and metabolic disease research requiring precise bioorthogonal labeling.