Met-Gly-Pro-AMC

• CAT Number: I040410
• CAS Number: 1926163-52-1
• Molecular Formula: C22H28N4O5S
• Molecular Weight: 460.55
• Purity: ≥95%
• Synonyms: (2S)-1-[2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]acetyl]-N-(4-methyl-2-oxochromen-7-yl)pyrrolidine-2-carboxamide
• InChI: InChI=1S/C22H28N4O5S/c1-13-10-20(28)31-18-11-14(5-6-15(13)18)25-22(30)17-4-3-8-26(17)19(27)12-24-21(29)16(23)7-9-32-2/h5-6,10-11,16-17H,3-4,7-9,12,23H2,1-2H3,(H,24,29)(H,25,30)/t16-,17-/m0/s1
• InChIKey: SBIKNEVHKGLANK-IRXDYDNUSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C3CCCN3C(=O)CNC(=O)C(CCSC)N

Met-Gly-Pro-AMC(CAT: I040410) is a synthetic fluorogenic peptide substrate designed for the sensitive detection of prolyl endopeptidase (PEP) activity. Upon enzymatic cleavage at the proline residue, the substrate releases 7-amino-4-methylcoumarin (AMC), a fluorescent molecule that enables real-time monitoring of enzyme kinetics. This substrate is widely used in studies of neurodegenerative diseases, cognitive function, and peptide hormone regulation, as PEP is involved in the processing of neuropeptides and peptide hormones. The Met-Gly-Pro sequence provides enzyme specificity, while the AMC tag ensures high sensitivity. Supplied at high purity, Met-Gly-Pro-AMC is ideal for applications in enzymology, drug screening, and neurobiology research.