Mal-amido-PEG8-NHS ester

• CAT Number: I016720
• CAS Number: 756525-93-6
• Molecular Formula: C30H47N3O15
• Molecular Weight: 689.71
• Purity: ≥95%
• Synonyms: (2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[2-[2-[2-[2-[3-(2,5-dioxopyrrol-1-yl)propanoylamino]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate
• InChI: InChI=1S/C30H47N3O15/c34-25(5-8-32-26(35)1-2-27(32)36)31-7-10-41-12-14-43-16-18-45-20-22-47-24-23-46-21-19-44-17-15-42-13-11-40-9-6-30(39)48-33-28(37)3-4-29(33)38/h1-2H,3-24H2,(H,31,34)
• InChIKey: MTPWCXBUZLEBDD-UHFFFAOYSA-N
• SMILES: C1CC(=O)N(C1=O)OC(=O)CCOCCOCCOCCOCCOCCOCCOCCOCCNC(=O)CCN2C(=O)C=CC2=O

Mal-amido-PEG8-NHS ester(CAT: I016720) is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring a maleimide group and an NHS ester, separated by an eight-unit PEG spacer. The NHS ester reacts efficiently with primary amines (–NH₂) on proteins, peptides, or biomolecules, while the maleimide selectively couples to sulfhydryl (–SH) groups on cysteines. The PEG8 chain improves water solubility, biocompatibility, and reduces steric hindrance, enabling flexible and stable conjugation. This reagent is widely used in preparing antibody–drug conjugates (ADCs), protein labeling, surface modification, and biomaterial functionalization. Mal-amido-PEG8-NHS ester provides a versatile, reliable tool for bioconjugation in chemical biology, diagnostics, and therapeutic research applications.