For research use only. Not for therapeutic Use.
L-Leucyl-L-Leucine methyl ester hydrochloride (Cat No.: R065177) is a lysosomotropic dipeptide methyl ester widely used to induce lysosomal membrane permeabilization (LMP) in cell biology research. Once internalized, it is hydrolyzed by lysosomal enzymes into membrane-disruptive products, causing lysosomal rupture and triggering downstream cell death pathways such as apoptosis or necrosis. The hydrochloride salt improves water solubility and handling. LLOMe is an important experimental tool for studying autophagy, lysosomal stability, immune cell cytotoxicity, and the molecular mechanisms underlying lysosome-dependent cell death in various disease models.
CAS Number | 6491-83-4 |
Synonyms | methyl (2S)-2-[[(2S)-2-amino-4-methylpentanoyl]amino]-4-methylpentanoate;hydrochloride |
Molecular Formula | C13H27ClN2O3 |
Purity | ≥95% |
InChI | InChI=1S/C13H26N2O3.ClH/c1-8(2)6-10(14)12(16)15-11(7-9(3)4)13(17)18-5;/h8-11H,6-7,14H2,1-5H3,(H,15,16);1H/t10-,11-;/m0./s1 |
InChIKey | RVXQFTNCULOWRV-ACMTZBLWSA-N |
SMILES | CC(C)CC(C(=O)NC(CC(C)C)C(=O)OC)N.Cl |
Reference | [1]. Thiele DL, et, al. The immunosuppressive activity of L-leucyl-L-leucine methyl ester: selective ablation of cytotoxic lymphocytes and monocytes. J Immunol. 1986 Feb 1;136(3):1038-48. [2]. Thiele DL, et, al. Mechanism of L-leucyl-L-leucine methyl ester-mediated killing of cytotoxic lymphocytes: dependence on a lysosomal thiol protease, dipeptidyl peptidase I, that is enriched in these cells. Proc Natl Acad Sci U S A. 1990 Jan;87(1):83-7. [3]. Kalogeropulou AF, et, al. Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity. Biochem J. 2020 Nov 27;477(22):4397-4423. |
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