L-Leucyl-L-Leucine methyl ester hydrobromide

• CAT Number: M079400
• CAS Number: 16689-14-8
• Molecular Formula: C13H27BrN2O3
• Molecular Weight: 339.27
• Purity: ≥95%
• Synonyms: methyl (2S)-2-[[(2S)-2-amino-4-methylpentanoyl]amino]-4-methylpentanoate;hydrobromide
• InChI: InChI=1S/C13H26N2O3.BrH/c1-8(2)6-10(14)12(16)15-11(7-9(3)4)13(17)18-5;/h8-11H,6-7,14H2,1-5H3,(H,15,16);1H/t10-,11-;/m0./s1
• InChIKey: QIPBCIHWFATZOF-ACMTZBLWSA-N
• SMILES: CC(C)CC(C(=O)NC(CC(C)C)C(=O)OC)N.Br

L-Leucyl-L-Leucine methyl ester hydrobromide (Cat No.: M079400) is a lysosomotropic dipeptide methyl ester used to selectively permeabilize lysosomal membranes in biological research. Once inside lysosomes, it is hydrolyzed to form membrane-disruptive products, triggering lysosomal rupture and cell death. LLOMe is widely employed to study lysosomal membrane permeabilization (LMP), autophagy, and cell death pathways, including apoptosis and necrosis. The hydrobromide salt form enhances solubility and stability. It serves as a valuable tool in immunology, cancer biology, and cell stress response investigations involving lysosomal function.

Reference

[1]. Thiele DL, et, al. The immunosuppressive activity of L-leucyl-L-leucine methyl ester: selective ablation of cytotoxic lymphocytes and monocytes. J Immunol. 1986 Feb 1;136(3):1038-48.
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[2]. Thiele DL, et, al. Mechanism of L-leucyl-L-leucine methyl ester-mediated killing of cytotoxic lymphocytes: dependence on a lysosomal thiol protease, dipeptidyl peptidase I, that is enriched in these cells. Proc Natl Acad Sci U S A. 1990 Jan;87(1):83-7.
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[3]. Kalogeropulou AF, et, al. Endogenous Rab29 does not impact basal or stimulated LRRK2 pathway activity. Biochem J. 2020 Nov 27;477(22):4397-4423.
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