Janelia Fluor® 549, Azide

• CAT Number: I045831
• Molecular Formula: C35H38N6O7
• Molecular Weight: 654.71
• Purity: ≥95%
• Synonyms: 2-[3-(azetidin-1-ium-1-ylidene)-6-(azetidin-1-yl)xanthen-9-yl]-4-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethylcarbamoyl]benzoate
• InChI: InChI=1S/C35H38N6O7/c36-39-38-10-16-46-18-20-47-19-17-45-15-9-37-34(42)24-3-6-27(35(43)44)30(21-24)33-28-7-4-25(40-11-1-12-40)22-31(28)48-32-23-26(5-8-29(32)33)41-13-2-14-41/h3-8,21-23H,1-2,9-20H2,(H-,37,42,43,44)
• InChIKey: XDSXVSGQYCACTI-UHFFFAOYSA-N
• SMILES: C1CN(C1)C2=CC3=C(C=C2)C(=C4C=CC(=[N+]5CCC5)C=C4O3)C6=C(C=CC(=C6)C(=O)NCCOCCOCCOCCN=[N+]=[N-])C(=O)[O-]

Janelia Fluor® 549, Azide（Cat No.:I045831）is a bright, photostable fluorescent dye designed for advanced bioimaging applications. With excitation and emission maxima near 549 nm, it offers high signal intensity and minimal photobleaching, making it ideal for super-resolution microscopy and live-cell imaging. The azide functional group enables copper-catalyzed or strain-promoted click chemistry, allowing covalent labeling of alkyne-containing biomolecules with high specificity. Its excellent cell permeability and low background fluorescence provide superior imaging quality, supporting studies in protein tracking, cellular dynamics, and biomolecular interactions in complex biological systems.