For research use only. Not for therapeutic Use.
Janelia Fluor® 525, SE（Cat No.:I045772）is a bright green fluorescent dye equipped with an NHS ester (SE) reactive group for efficient covalent labeling of primary amines in proteins, peptides, and other biomolecules. With excitation and emission maxima around 516/525 nm, it offers high quantum yield, strong photostability, and minimal background, making it ideal for fluorescence microscopy, flow cytometry, and imaging assays. Its spectral properties complement red and far-red dyes, enabling multiplexed experiments. Janelia Fluor® 525, SE is widely applied in live-cell imaging, protein tagging, and advanced fluorescence-based biological studies.
| Synonyms | 2-[3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)xanthen-9-yl]-4-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate |
| Molecular Formula | C31H21F4N3O7 |
| Purity | ≥95% |
| IUPAC Name | 2-[3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)xanthen-9-yl]-4-(2,5-dioxopyrrolidin-1-yl)oxycarbonylbenzoate |
| InChI | InChI=1S/C31H21F4N3O7/c32-30(33)12-36(13-30)17-2-5-20-23(10-17)44-24-11-18(37-14-31(34,35)15-37)3-6-21(24)27(20)22-9-16(1-4-19(22)28(41)42)29(43)45-38-25(39)7-8-26(38)40/h1-6,9-11H,7-8,12-15H2 |
| InChIKey | VPHPPEABRFKVDC-UHFFFAOYSA-N |
| SMILES | C1CC(=O)N(C1=O)OC(=O)C2=CC(=C(C=C2)C(=O)[O-])C3=C4C=CC(=[N+]5CC(C5)(F)F)C=C4OC6=C3C=CC(=C6)N7CC(C7)(F)F |
| Chemistry Calculators | Dilution Calculator In vivo Formulation Calculator Molarity Calculator Molecular Weight Calculator Reconstitution Calculator |
