HKYellow-AM (6/12-mixture)

• CAT Number: I045486
• CAS Number: 1448821-89-3
• Molecular Formula: C80H78Cl2N4O16
• Molecular Weight: 1422.40
• Purity: ≥95%
• Synonyms: acetyloxymethyl 4-[8′-chloro-9′-(4-hydroxy-N-methylanilino)-2′,2′,4′-trimethyl-3-oxospiro[2-benzofuran-1,6′-3,4-dihydrochromeno[3,2-g]quinoline]-1′-yl]butanoate;acetyloxymethyl 4-[10′-chloro-9′-(4-hydroxy-N-methylanilino)-2′,2′,4′-trimethyl-3-oxospiro[2-benzofuran-1,12′-3,4-dihydrochromeno[2,3-h]quinoline]-1′-yl]butanoate
• InChI: InChI=1S/2C40H39ClN2O8/c1-23-21-39(3,4)43(18-8-11-35(46)49-22-48-24(2)44)37-27(23)16-17-33-36(37)40(29-10-7-6-9-28(29)38(47)51-40)30-19-31(41)32(20-34(30)50-33)42(5)25-12-14-26(45)15-13-25;1-23-21-39(3,4)43(16-8-11-37(46)49-22-48-24(2)44)33-19-35-30(17-28(23)33)40(29-10-7-6-9-27(29)38(47)51-40)31-18-32(41)34(20-36(31)50-35)42(5)25-12-14-26(45)15-13-25/h6-7,9-10,12-17,19-20,23,45H,8,11,18,21-22H2,1-5H3;6-7,9-10,12-15,17-20,23,45H,8,11,16,21-22H2,1-5H3
• InChIKey: WWDXCCHKVDGIDU-UHFFFAOYSA-N
• SMILES: CC1CC(N(C2=C1C=CC3=C2C4(C5=CC=CC=C5C(=O)O4)C6=CC(=C(C=C6O3)N(C)C7=CC=C(C=C7)O)Cl)CCCC(=O)OCOC(=O)C)(C)C.CC1CC(N(C2=CC3=C(C=C12)C4(C5=CC=CC=C5C(=O)O4)C6=CC(=C(C=C6O3)N(C)C7=CC=C(C=C7)O)Cl)CCCC(=O)OCOC(=O)C)(C)C

HKYellow-AM (Cat No.: I045486) is a cell-permeable fluorescent probe designed for live-cell imaging of hydrogen peroxide (H₂O₂). The acetoxymethyl (AM) ester groups enable efficient membrane penetration, after which intracellular esterases hydrolyze them to release the active HKYellow dye. Upon reacting with H₂O₂, the probe emits a bright yellow fluorescence, allowing real-time visualization of oxidative signaling and stress. HKYellow-AM is widely used in redox biology, cell signaling, and disease research to study H₂O₂ dynamics with high sensitivity, selectivity, and compatibility for live-cell fluorescence microscopy.

Reference

[1]. Trends Cell Biol. 2007 Sep;17(9):422-7. doi: 10.1016/j.tcb.2007.07.009. Epub 2007 Sep 4.
[Content Brief]

[2]. Yang D, et, al. Diarylamine-based fluorogenic probes for detection of peroxynitrite. EP2809666B1.

[3]. Yang D, et, al. Diarylamine-based fluorogenic probes for detection of peroxynitrite. EP2809666B1.