HADA hydrochloride

• CAT Number: I045472
• CAS Number: 2253733-10-5
• Molecular Formula: C13H13ClN2O6
• Molecular Weight: 328.71
• Purity: ≥95%
• Synonyms: (2R)-2-amino-3-[(7-hydroxy-2-oxochromene-3-carbonyl)amino]propanoic acid;hydrochloride
• InChI: InChI=1S/C13H12N2O6.ClH/c14-9(12(18)19)5-15-11(17)8-3-6-1-2-7(16)4-10(6)21-13(8)20;/h1-4,9,16H,5,14H2,(H,15,17)(H,18,19);1H/t9-;/m1./s1
• InChIKey: PLVOEKQNBIXHDI-SBSPUUFOSA-N
• SMILES: C1=CC2=C(C=C1O)OC(=O)C(=C2)C(=O)NCC(C(=O)O)N.Cl

HADA hydrochloride (Cat No.: I045472) is a fluorescent dye used as a probe for β-galactosidase activity in biochemical assays. Upon enzymatic cleavage of its galactoside-conjugated form, HADA releases a highly fluorescent product, enabling sensitive detection of β-galactosidase in cells, tissues, or microbial cultures. The hydrochloride salt improves solubility and handling. HADA is widely applied in molecular biology, microbiology, and enzymology for reporter gene assays, metabolic studies, and high-throughput screening, offering high sensitivity, stability, and compatibility with fluorescence-based detection systems.

Reference

[1]. Erkin Kuru, et al. Synthesis of fluorescent D-amino acids and their use for probing peptidoglycan synthesis and bacterial growth in situ. Nat Protoc. 2015 Jan;10(1):33-52.
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