H-D-CHA-Ala-Arg-pNA diacetate

• CAT Number: I040195
• Molecular Formula: C28H46N8O9
• Molecular Weight: 638.71
• Purity: ≥95%
• Synonyms: acetic acid;(2S)-2-[[(2S)-2-[[(2R)-2-amino-3-cyclohexylpropanoyl]amino]propanoyl]amino]-5-(diaminomethylideneamino)-N-(4-nitrophenyl)pentanamide
• InChI: InChI=1S/C24H38N8O5.2C2H4O2/c1-15(29-22(34)19(25)14-16-6-3-2-4-7-16)21(33)31-20(8-5-13-28-24(26)27)23(35)30-17-9-11-18(12-10-17)32(36)37;2*1-2(3)4/h9-12,15-16,19-20H,2-8,13-14,25H2,1H3,(H,29,34)(H,30,35)(H,31,33)(H4,26,27,28);2*1H3,(H,3,4)/t15-,19+,20-;;/m0../s1
• InChIKey: GOOYGVMAIMOCLQ-AWFQOOCHSA-N
• SMILES: CC(C(=O)NC(CCCN=C(N)N)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)C(CC2CCCCC2)N.CC(=O)O.CC(=O)O

H-D-CHA-Ala-Arg-pNA (diacetate)(CAT: I040195) is the diacetate salt form of H-D-CHA-Ala-Arg-pNA (HY-150523) and serves as a chromogenic substrate for amidolytic enzyme assays. This synthetic peptide releases p-nitroaniline (pNA) upon enzymatic cleavage, producing a measurable yellow color that enables quantitative spectrophotometric detection of protease activity. It is particularly useful for studying serine proteases and other enzymes involved in coagulation, inflammation, and fibrinolysis. The diacetate form enhances the compound’s solubility and handling in aqueous buffers. H-D-CHA-Ala-Arg-pNA (diacetate) is ideal for use in biochemical assays, enzyme kinetics, and drug screening applications where precise detection of proteolytic activity is essential.