GW 4869 dihydrocloride - CAS 6823-69-4

Neutral sphingomyelinases mediate the release of ceramide from sphingomyelin in cellular membranes and can be activated by certain stresses. Ceramide can act as a signaling molecule in its own right, or it can be further processed to generate sphingosine (and sphingosine-1-phosphate). GW 4869 is a cell-permeable, non-competitive inhibitor of neutral sphingomyelinases (IC50 = 1 μM), but does not affect acid sphingomyelinase activity. It inhibits TNF-α-mediated sphingomyelin hydrolysis (100% inhibition at 20 μM) and TNF-α-induced cell death in MCF-7 cells without interfering with activation of NF-κB or glutathione depletion in response to TNF. GW 4869 also reduces the inhibitory effect of oxidized phospholipid products on lipopolysaccharides-mediated induction of interleukin-8 in human aortic endothelial cells and prevents hypoxia-induced pulmonary vasoconstriction in rats in vivo.

Catalog Number: M024107

CAS Number: 6823-69-4

PubChem Substance ID:355036423

Molecular Formula: C₃₀H₂₈N₆O₂ · 2HCl [xH₂O]

Molecular Weight:577.5

Purity: ≥95%

* For research use only. Not for human or veterinary use.


SynonymsN-SMase Inhibitor;2-PropenaMide, 3,3/’-(1,4-phenylene)bis[N-[4-(4,5-dihydro-1H-iMidazol-2-yl)phenyl]-, hydrochloride (1:2);4/’,4/’/’-Di-2-imidazolin-2-yl-p-benzenediacrylanilide dihydrochloride;GW 4869 (hydrochloride hydrate)


Molecular Formula: C₃₀H₂₈N₆O₂ · 2HCl [xH₂O]
Molecular Weight577.5

Computed Descriptor