Gly-Arg-AMC

• CAT Number: I040411
• CAS Number: 65147-19-5
• Molecular Formula: C18H24N6O4
• Molecular Weight: 388.42
• Purity: ≥95%
• Synonyms: (2S)-2-[(2-aminoacetyl)amino]-5-(diaminomethylideneamino)-N-(4-methyl-2-oxochromen-7-yl)pentanamide
• InChI: InChI=1S/C18H24N6O4/c1-10-7-16(26)28-14-8-11(4-5-12(10)14)23-17(27)13(24-15(25)9-19)3-2-6-22-18(20)21/h4-5,7-8,13H,2-3,6,9,19H2,1H3,(H,23,27)(H,24,25)(H4,20,21,22)/t13-/m0/s1
• InChIKey: JNTASUHAFOHMQK-ZDUSSCGKSA-N
• SMILES: CC1=CC(=O)OC2=C1C=CC(=C2)NC(=O)C(CCCN=C(N)N)NC(=O)CN

Gly-Arg-AMC(CAT: I040411) is a fluorogenic dipeptide substrate designed for the sensitive detection of arginine-specific protease activity, particularly trypsin-like serine proteases. Upon enzymatic cleavage at the arginine residue, the substrate releases 7-amino-4-methylcoumarin (AMC), producing a measurable fluorescent signal ideal for real-time enzymatic assays. Its minimal dipeptide structure allows rapid substrate turnover and high assay sensitivity, making it valuable for enzyme kinetics, inhibitor screening, and high-throughput analysis. Gly-Arg-AMC is commonly used in biochemical and pharmacological studies involving protease function and regulation. Supplied at high purity, it is optimized for reliable use in protease profiling and activity-based enzyme assays.