Fluorescein tyramide

• CAT Number: I045475
• CAS Number: 210236-90-1
• Molecular Formula: C29H21NO7
• Molecular Weight: 495.49
• Purity: ≥95%
• Synonyms: 3′,6′-dihydroxyspiro[2-benzofuran-3,9′-xanthene]-1-one;N-[2-(4-hydroxyphenyl)ethyl]acetamide
• InChI: InChI=1S/C20H12O5.C10H13NO2/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;1-8(12)11-7-6-9-2-4-10(13)5-3-9/h1-10,21-22H;2-5,13H,6-7H2,1H3,(H,11,12)
• InChIKey: RIHCPYWHAYBEHO-UHFFFAOYSA-N
• SMILES: CC(=O)NCCC1=CC=C(C=C1)O.C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O

Fluorescein tyramide (Cat No.: I045475) is a fluorescent labeling reagent used in tyramide signal amplification (TSA), a highly sensitive detection method for biomolecules in immunohistochemistry, in situ hybridization, and ELISA. Upon activation by horseradish peroxidase (HRP), fluorescein tyramide forms reactive radicals that covalently bind to nearby tyrosine residues on proteins, enabling stable and localized signal deposition. The fluorescein moiety provides bright green fluorescence for microscopic visualization. This reagent is widely applied in diagnostics and research to achieve high-resolution, low-background detection of target molecules in complex biological samples.

Reference

[1]. A H Hopman, et al. Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for In situ hybridization using CARD amplification. J Histochem Cytochem. 1998 Jun;46(6):771-7.
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[2]. R P van Gijlswijk, et al. Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem. 1997 Mar;45(3):375-82.
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