Fluorescein-DBCO

• CAT Number: I016399
• CAS Number: 2054339-00-1
• Molecular Formula: C39H27N3O6S
• Molecular Weight: 665.71
• Purity: ≥95%
• Synonyms: 1-[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]-3-(3′,6′-dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-yl)thiourea
• IUPAC Name: 1-[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]-3-(3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-yl)thiourea
• InChI: InChI=1S/C39H27N3O6S/c43-27-12-15-31-34(20-27)47-35-21-28(44)13-16-32(35)39(31)30-14-11-26(19-29(30)37(46)48-39)41-38(49)40-18-17-36(45)42-22-25-7-2-1-5-23(25)9-10-24-6-3-4-8-33(24)42/h1-8,11-16,19-21,43-44H,17-18,22H2,(H2,40,41,49)
• InChIKey: FGEDVMJBPDYXLU-UHFFFAOYSA-N
• SMILES: C1C2=CC=CC=C2C#CC3=CC=CC=C3N1C(=O)CCNC(=S)NC4=CC5=C(C=C4)C6(C7=C(C=C(C=C7)O)OC8=C6C=CC(=C8)O)OC5=O

Fluorescein-DBCO(CAT: I016399) is a fluorescent dye conjugate that combines the bright green fluorophore fluorescein with a dibenzocyclooctyne (DBCO) moiety for copper-free click chemistry. The DBCO group reacts rapidly and selectively with azide-containing biomolecules through strain-promoted alkyne–azide cycloaddition (SPAAC), eliminating the need for toxic copper catalysts and preserving biological activity. The fluorescein component provides strong excitation/emission properties (Ex ~495 nm, Em ~520 nm), making it ideal for fluorescence imaging, microscopy, and flow cytometry applications. This reagent is widely applied in biomolecule labeling, live-cell imaging, and tracking of azide-modified proteins, glycans, or nucleic acids in bioconjugation and bioorthogonal research.