FITC

• CAT Number: R001178
• CAS Number: 3326-32-7
• Molecular Formula: C21H11NO5S
• Molecular Weight: 389.38
• Purity: ≥95%
• Synonyms: 3′,6′-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9′-xanthene]-1-one
• InChI: InChI=1S/C21H11NO5S/c23-12-2-5-16-18(8-12)26-19-9-13(24)3-6-17(19)21(16)15-4-1-11(22-10-28)7-14(15)20(25)27-21/h1-9,23-24H
• InChIKey: MHMNJMPURVTYEJ-UHFFFAOYSA-N
• SMILES: C1=CC2=C(C=C1N=C=S)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O

FFluorescein isothiocyanate (Cat No.: R001178) is a widely used fluorescent dye that covalently binds to proteins and other biomolecules via its reactive isothiocyanate group. It emits bright green fluorescence when excited at around 495 nm, making it ideal for applications such as flow cytometry, immunofluorescence, and fluorescence microscopy. FITC-labeled antibodies are commonly used for detecting specific antigens. Despite its photobleaching sensitivity, FITC remains a popular choice due to its compatibility with standard fluorescence filters and broad availability in conjugated forms.

Reference

[1]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.
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[2]. Gao SG, et al. Phosphorylation of osteopontin has proapoptotic and proinflammatory effects on human knee osteoarthritis chondrocytes. Exp Ther Med. 2016 Nov;12(5):3488-3494. Epub 2016 Oct 5.
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[3]. Zhu X, et al. Ratiometric, visual, dual-signal fluorescent sensing and imaging of pH/copper ions in real samples based on carbon dots-fluorescein isothiocyanate composites. Talanta. 2017 Jan 1;162:65-71
[Content Brief]