DFHBI-2T

• CAT Number: I045634
• CAS Number: 1539318-40-5
• Molecular Formula: C12H7F5N2O2
• Molecular Weight: 306.19
• Purity: ≥95%
• Synonyms: (5Z)-5-[(3,5-difluoro-4-hydroxyphenyl)methylidene]-3-methyl-2-(trifluoromethyl)imidazol-4-one
• InChI: InChI=1S/C12H7F5N2O2/c1-19-10(21)8(18-11(19)12(15,16)17)4-5-2-6(13)9(20)7(14)3-5/h2-4,20H,1H3/b8-4-
• InChIKey: CGFZNPPTJGGWPN-YWEYNIOJSA-N
• SMILES: CN1C(=O)C(=CC2=CC(=C(C(=C2)F)O)F)N=C1C(F)(F)F

DFHBI-2T (Cat No.: I045634) is a fluorogenic dye widely used in RNA imaging studies as the primary ligand for RNA aptamers such as Spinach and Broccoli. Upon binding to these engineered RNA sequences, DFHBI-2T fluoresces brightly, enabling real-time visualization of RNA dynamics in living cells. Its stability and enhanced photophysical properties make it superior to earlier analogs, supporting long-term imaging applications. Researchers use DFHBI-2T to investigate RNA localization, transcription, and processing. While not therapeutic, it is a valuable tool in molecular and cellular biology.

Reference

[1]. Wenjiao Song, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201.
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[2]. Robert J Trachman 3rd, et al. Structural Principles of Fluorescent RNA Aptamers. Trends Pharmacol Sci. 2017 Oct;38(10):928-939.
[Content Brief]