DFHBI-1T

• CAT Number: I011255
• CAS Number: 1539318-36-9
• Molecular Formula: C13H9F5N2O2
• Molecular Weight: 320.21
• Purity: ≥95%
• Synonyms: (5Z)-5-[(3,5-difluoro-4-hydroxyphenyl)methylidene]-2-methyl-3-(2,2,2-trifluoroethyl)imidazol-4-one
• InChI: InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-
• InChIKey: AWYCLBWNRONMQC-WMZJFQQLSA-N
• SMILES: CC1=NC(=CC2=CC(=C(C(=C2)F)O)F)C(=O)N1CC(F)(F)F

DFHBI-1T (Cat No.: I011255) is a synthetic fluorogenic dye designed to bind RNA aptamers such as Spinach and Broccoli, enabling fluorescence-based visualization of RNA molecules in live cells. Upon binding, it emits strong green fluorescence, making it a useful tool for tracking RNA localization, dynamics, and expression in real time. Compared with earlier derivatives, DFHBI-1T offers improved brightness and stability, enhancing its performance in imaging applications. Though not a therapeutic agent, DFHBI-1T is widely applied in molecular biology and RNA research.

Reference

[1]. Wenjiao Song, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201.
[Content Brief]

[2]. Grigory S Filonov, et al. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. J Am Chem Soc. 2014 Nov 19;136(46):16299-308.
[Content Brief]