DBCO-PEG4-Maleimide

• CAT Number: I017173
• CAS Number: 1480516-75-3
• Molecular Formula: C36H42N4O9
• Molecular Weight: 674.74
• Purity: ≥95%
• Synonyms: N-[2-[2-[2-[2-[3-[[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]amino]-3-oxopropoxy]ethoxy]ethoxy]ethoxy]ethyl]-3-(2,5-dioxopyrrol-1-yl)propanamide
• InChI: InChI=1S/C36H42N4O9/c41-32(14-18-39-34(43)11-12-35(39)44)38-17-20-47-22-24-49-26-25-48-23-21-46-19-15-33(42)37-16-13-36(45)40-27-30-7-2-1-5-28(30)9-10-29-6-3-4-8-31(29)40/h1-8,11-12H,13-27H2,(H,37,42)(H,38,41)
• InChIKey: VVFZXPZWVJMYPX-UHFFFAOYSA-N
• SMILES: C1C2=CC=CC=C2C#CC3=CC=CC=C3N1C(=O)CCNC(=O)CCOCCOCCOCCOCCNC(=O)CCN4C(=O)C=CC4=O

DBCO-PEG4-Maleimide(CAT:I017173) is a heterobifunctional PEG-based crosslinker that integrates a dibenzocyclooctyne (DBCO) group with a maleimide functionality, separated by a four-unit PEG spacer. The DBCO group enables copper-free strain-promoted azide–alkyne cycloaddition (SPAAC) for rapid, bioorthogonal click chemistry with azide-containing molecules, while the maleimide moiety selectively reacts with sulfhydryl (–SH) groups on proteins and peptides. The PEG4 linker enhances solubility, flexibility, and minimizes steric hindrance, ensuring efficient conjugation in aqueous systems. DBCO-PEG4-Maleimide is widely used in bioconjugation, antibody–drug conjugates (ADCs), protein labeling, surface functionalization, and drug delivery research, providing precise, stable, and site-specific biomolecule modification.