DBCO-NHCO-S-S-NHS ester

• CAT Number: I045956
• Molecular Formula: C28H27N3O6S2
• Molecular Weight: 565.66
• Purity: ≥95%
• Synonyms: (2,5-dioxopyrrolidin-1-yl) 3-[[3-[[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]amino]-3-oxopropyl]disulfanyl]propanoate
• InChI: InChI=1S/C28H27N3O6S2/c32-24(14-17-38-39-18-15-28(36)37-31-26(34)11-12-27(31)35)29-16-13-25(33)30-19-22-7-2-1-5-20(22)9-10-21-6-3-4-8-23(21)30/h1-8H,11-19H2,(H,29,32)
• InChIKey: PFCJWHZTYTWKNS-UHFFFAOYSA-N
• SMILES: C1CC(=O)N(C1=O)OC(=O)CCSSCCC(=O)NCCC(=O)N2CC3=CC=CC=C3C#CC4=CC=CC=C42

DBCO-NHCO-S-S-NHS ester(CAT: I045956) is a cleavable, heterobifunctional bioconjugation reagent featuring a dibenzocyclooctyne (DBCO) group, a disulfide linkage, and an NHS-activated ester. The DBCO moiety enables copper-free strain-promoted alkyne–azide cycloaddition (SPAAC) with azide-containing biomolecules, ensuring rapid and biocompatible conjugation. The NHS ester reacts efficiently with primary amines on peptides, proteins, or ligands to form stable amide bonds. The incorporated disulfide bond provides a cleavable linker, allowing controlled release of conjugated molecules under reducing conditions. This multifunctional design makes DBCO-NHCO-S-S-NHS ester a powerful tool for developing antibody–drug conjugates, inhibitors, and bioorthogonal probes in cancer, immunology, and targeted drug delivery research.