DBCO-NHCO-PEG6-Biotin

• CAT Number: I045948
• Molecular Formula: C43H59N5O10S
• Molecular Weight: 838.02
• Purity: ≥95%
• Synonyms: N-[2-[2-[2-[2-[2-[2-[3-[[3-(2-azatricyclo[10.4.0.04,9]hexadeca-1(16),4,6,8,12,14-hexaen-10-yn-2-yl)-3-oxopropyl]amino]-3-oxopropoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethyl]-5-[(4R)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanamide
• InChI: InChI=1S/C43H59N5O10S/c49-39(12-6-5-11-38-42-36(32-59-38)46-43(52)47-42)45-18-20-54-22-24-56-26-28-58-30-29-57-27-25-55-23-21-53-19-16-40(50)44-17-15-41(51)48-31-35-9-2-1-7-33(35)13-14-34-8-3-4-10-37(34)48/h1-4,7-10,36,38,42H,5-6,11-12,15-32H2,(H,44,50)(H,45,49)(H2,46,47,52)/t36?,38-,42?/m1/s1
• InChIKey: VSRSCDHOVWPTLX-ZUFGXVEESA-N
• SMILES: C1C2C([C@H](S1)CCCCC(=O)NCCOCCOCCOCCOCCOCCOCCC(=O)NCCC(=O)N3CC4=CC=CC=C4C#CC5=CC=CC=C53)NC(=O)N2

DBCO-NHCO-PEG6-Biotin（Cat No.:I045948）is a multifunctional, biotinylated linker optimized for bioorthogonal labeling and detection. The DBCO (dibenzocyclooctyne) group enables strain-promoted azide–alkyne cycloaddition (SPAAC), allowing rapid, copper-free click reactions with azides under biocompatible conditions. The PEG6 spacer improves aqueous solubility, reduces steric hindrance, and adds flexibility, ensuring efficient conjugation in complex biomolecular systems. The NHCO linkage provides stability, while the biotin moiety offers strong affinity for avidin/streptavidin binding, supporting detection, enrichment, and assay development. This reagent is widely applied in chemical biology, proteomics, imaging, and drug delivery research requiring selective and efficient biotinylation.