D-Pro-Phe-Arg-Chloromethylketone

• CAT Number: I040414
• CAS Number: 88546-74-1
• Molecular Formula: C21H31ClN6O3
• Molecular Weight: 450.96
• Purity: ≥95%
• Synonyms: (2R)-N-[(2S)-1-[[(3S)-1-chloro-6-(diaminomethylideneamino)-2-oxohexan-3-yl]amino]-1-oxo-3-phenylpropan-2-yl]pyrrolidine-2-carboxamide
• InChI: InChI=1S/C21H31ClN6O3/c22-13-18(29)15(8-4-11-26-21(23)24)27-20(31)17(12-14-6-2-1-3-7-14)28-19(30)16-9-5-10-25-16/h1-3,6-7,15-17,25H,4-5,8-13H2,(H,27,31)(H,28,30)(H4,23,24,26)/t15-,16+,17-/m0/s1
• InChIKey: HCCRRLVJBLDSLL-BBWFWOEESA-N
• SMILES: C1CC(NC1)C(=O)NC(CC2=CC=CC=C2)C(=O)NC(CCCN=C(N)N)C(=O)CCl

D-Pro-Phe-Arg-Chloromethylketone (D-Phe-PR-CMK)(CAT: I040414) is a synthetic tripeptide-based irreversible inhibitor targeting serine proteases, particularly thrombin and factor Xa. Featuring a chloromethyl ketone reactive group, it covalently binds to active site serine residues, resulting in permanent enzymatic inactivation. The D-proline configuration enhances resistance to proteolytic degradation, making it a stable and effective tool in coagulation research, enzyme mechanism studies, and protease profiling. This inhibitor is frequently used in the study of blood clotting, thrombosis, and cardiovascular biology. Supplied at high purity, D-Pro-Phe-Arg-CMK is suitable for advanced biochemical and pharmaceutical research involving targeted protease inhibition.