Cy5-PEG3-azide

• CAT Number: I025317
• Molecular Formula: C40H55ClN6O4
• Molecular Weight: 719.36
• Purity: ≥95%
• Synonyms: N-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethyl]-6-[(2E)-3,3-dimethyl-2-[(2E,4E)-5-(1,3,3-trimethylindol-1-ium-2-yl)penta-2,4-dienylidene]indol-1-yl]hexanamide;chloride
• InChI: InChI=1S/C40H54N6O4.ClH/c1-39(2)32-16-11-13-18-34(32)45(5)36(39)20-8-6-9-21-37-40(3,4)33-17-12-14-19-35(33)46(37)25-15-7-10-22-38(47)42-23-26-48-28-30-50-31-29-49-27-24-43-44-41;/h6,8-9,11-14,16-21H,7,10,15,22-31H2,1-5H3;1H
• InChIKey: IPFARVGHBHWPPX-UHFFFAOYSA-N
• SMILES: CC1(C2=CC=CC=C2[N+](=C1/C=C/C=C/C=C/3\C(C4=CC=CC=C4N3CCCCCC(=O)NCCOCCOCCOCCN=[N+]=[N-])(C)C)C)C.[Cl-]

Cy5-PEG3-azide（Cat No.:I025317）is a far-red fluorescent probe that combines the Cy5 dye with a PEG3 linker and a terminal azide group for bioorthogonal labeling. The azide functionality enables efficient copper-catalyzed (CuAAC) or copper-free click reactions with alkyne- or DBCO-modified biomolecules, allowing site-specific conjugation. The PEG3 spacer enhances solubility, flexibility, and reduces steric hindrance, improving labeling efficiency in biological environments. With its bright far-red emission, low background, and excellent photostability, Cy5-PEG3-azide is widely used in fluorescence microscopy, proteomics, and live-cell imaging for biomolecular tracking and visualization.