Compound E

• CAT Number: R065078
• CAS Number: 209986-17-4
• Molecular Formula: C₂₇H₂₄F₂N₄O₃
• Molecular Weight: 490.51
• Purity: ≥95%
• Synonyms: γ-Secretase Inhibitor XXI
• Target: Stem Cell/Wnt
• Storage: -20°C
• IUPAC Name: (2S)-2-[[2-(3,5-difluorophenyl)acetyl]amino]-N-[(3S)-1-methyl-2-oxo-5-phenyl-3H-1,4-benzodiazepin-3-yl]propanamide
• InChI: InChI=1S/C27H24F2N4O3/c1-16(30-23(34)14-17-12-19(28)15-20(29)13-17)26(35)32-25-27(36)33(2)22-11-7-6-10-21(22)24(31-25)18-8-4-3-5-9-18/h3-13,15-16,25H,14H2,1-2H3,(H,30,34)(H,32,35)/t16-,25+/m0/s1
• InChIKey: JNGZXGGOCLZBFB-IVCQMTBJSA-N
• SMILES: CC(C(=O)NC1C(=O)N(C2=CC=CC=C2C(=N1)C3=CC=CC=C3)C)NC(=O)CC4=CC(=CC(=C4)F)F

Compound E(CAT: R065078) is a potent, selective, and cell-permeable γ-secretase inhibitor with an IC₅₀ of approximately 0.3 nM. γ-Secretase is a multimeric aspartyl protease that regulates key signaling pathways by cleaving membrane proteins such as Notch and amyloid precursor protein (APP). Compound E effectively blocks the proteolytic processing of these substrates, thereby suppressing Notch signaling and APP cleavage. As a result, it induces neuronal differentiation, impairs ovarian folliculogenesis, and inhibits thymocyte development. Widely used in developmental biology and neurodegenerative disease research, Compound E serves as a critical tool for studying the molecular mechanisms of Alzheimer’s disease and Notch-related pathways.