CNX-500

• CAT Number: I017271
• CAS Number: 1202758-21-1
• Molecular Formula: C48H68N10O9S
• Molecular Weight: 961.18
• Purity: ≥95%
• Synonyms: N-[3-[2-[2-[3-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]propoxy]ethoxy]ethoxy]propyl]-N’-[3-[3-[[5-methyl-4-[3-(prop-2-enoylamino)anilino]pyrimidin-2-yl]amino]phenoxy]propyl]pentanediamide
• IUPAC Name: N-[3-[2-[2-[3-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]propoxy]ethoxy]ethoxy]propyl]-N'-[3-[3-[[5-methyl-4-[3-(prop-2-enoylamino)anilino]pyrimidin-2-yl]amino]phenoxy]propyl]pentanediamide
• InChI: InChI=1S/C48H68N10O9S/c1-3-41(59)53-35-12-6-13-36(30-35)54-46-34(2)32-52-47(58-46)55-37-14-7-15-38(31-37)67-25-11-22-51-44(62)19-8-18-43(61)50-21-10-24-65-27-29-66-28-26-64-23-9-20-49-42(60)17-5-4-16-40-45-39(33-68-40)56-48(63)57-45/h3,6-7,12-15,30-32,39-40,45H,1,4-5,8-11,16-29,33H2,2H3,(H,49,60)(H,50,61)(H,51,62)(H,53,59)(H2,56,57,63)(H2,52,54,55,58)/t39-,40-,45-/m0/s1
• InChIKey: SBOPEFHTOFMNSD-IAWMPOBQSA-N
• SMILES: CC1=CN=C(N=C1NC2=CC(=CC=C2)NC(=O)C=C)NC3=CC(=CC=C3)OCCCNC(=O)CCCC(=O)NCCCOCCOCCOCCCNC(=O)CCCCC4C5C(CS4)NC(=O)N5

CNX-500(CAT: I017271) is a biotinylated covalent probe designed from the selective Bruton’s tyrosine kinase (Btk) inhibitor CC-292. It retains potent inhibitory activity against Btk, with an IC₅₀ of 0.5 nM, and forms stable covalent bonds with the kinase. CNX-500 displays minimal off-target inhibition of EGFR and Src-family kinases, including Syk and Lyn. In competition assays, the probe enables direct detection of free, uninhibited Btk, as it cannot bind to Btk already occupied by CC-292. In Ramos cells, CNX-500 quantifies Btk occupancy in proportion to CC-292 concentrations, making it a valuable tool for studying Btk engagement and drug-target interactions.