CM-H2DCFDA

• CAT Number: I042559
• CAS Number: 850013-49-9
• Molecular Formula: C27H19Cl3O8
• Molecular Weight: 577.79
• Purity: ≥95%
• Synonyms: acetyl 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoate;chloroethane
• InChI: InChI=1S/C26H18Cl2O8.C2H5Cl/c1-12(29)33-23-10-21-17(8-19(23)27)25(15-6-4-5-7-16(15)26(32)35-14(3)31)18-9-20(28)24(34-13(2)30)11-22(18)36-21;1-2-3/h4-11,25H,1-3H3;2H2,1H3
• InChIKey: JOMNIEDHXZVHEU-UHFFFAOYSA-N
• SMILES: CCCl.CC(=O)OC1=C(C=C2C(C3=CC(=C(C=C3OC2=C1)OC(=O)C)Cl)C4=CC=CC=C4C(=O)OC(=O)C)Cl

CM-H2DCFDA (Cat No.: I042559) is a cell-permeable fluorescent probe used to detect reactive oxygen species (ROS) in live cells. Once inside the cell, esterases deacetylate it to a non-fluorescent form, which is then oxidized by ROS to emit green fluorescence. The chloromethyl group allows for better retention within cells, enhancing imaging accuracy. CM-H2DCFDA is widely used in oxidative stress research, drug screening, and cell signaling studies to monitor intracellular ROS dynamics and assess antioxidant efficacy.

Reference

[1]. Oparka M, et al. Quantifying ROS levels using CM-H2DCFDA and HyPer. Methods. 2016 Oct 15;109:3-11.
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[2]. Kolarova H, et al. Production of reactive oxygen species after photodynamic therapy by porphyrin sensitizers. Gen Physiol Biophys. 2008 Jun;27(2):101-5.
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