Cathepsin D and E FRET Substrate acetate

• CAT Number: I044279
• CAS Number: 839730-93-7
• Molecular Formula: C87H126N22O21
• Molecular Weight: 1816.15
• Purity: ≥95%
• IUPAC Name: acetic acid;(2S)-N-[(2S,3S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2S)-1-[[(2R)-1-amino-5-carbamimidamido-1-oxopentan-2-yl]amino]-6-(2,4-dinitroanilino)-1-oxohexan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-carbamimidamido-1-oxopentan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-1-oxo-3-phenylpropan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-3-methyl-1-oxopentan-2-yl]-1-[(2S)-6-amino-2-[[2-[(7-methoxy-2-oxochromene-4-carbonyl)amino]acetyl]amino]hexanoyl]pyrrolidine-2-carboxamide
• InChI: InChI=1S/C84H120N22O19.C2H4O2/c1-8-50(6)71(103-80(117)66-30-21-39-104(66)82(119)61(27-15-17-35-85)95-69(107)47-94-73(110)56-46-70(108)125-68-45-54(124-7)32-33-55(56)68)81(118)102-63(41-49(4)5)77(114)100-65(43-52-24-13-10-14-25-52)79(116)101-64(42-51-22-11-9-12-23-51)78(115)98-60(29-20-38-93-84(89)90)75(112)99-62(40-48(2)3)76(113)97-59(74(111)96-58(72(86)109)28-19-37-92-83(87)88)26-16-18-36-91-57-34-31-53(105(120)121)44-67(57)106(122)123;1-2(3)4/h9-14,22-25,31-34,44-46,48-50,58-66,71,91H,8,15-21,26-30,35-43,47,85H2,1-7H3,(H2,86,109)(H,94,110)(H,95,107)(H,96,111)(H,97,113)(H,98,115)(H,99,112)(H,100,114)(H,101,116)(H,102,118)(H,103,117)(H4,87,88,92)(H4,89,90,93);1H3,(H,3,4)/t50-,58+,59-,60-,61-,62-,63-,64-,65-,66-,71-;/m0./s1
• InChIKey: JJLQDALOHFPDQU-YUPCDROESA-N
• SMILES: CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC2=CC=CC=C2)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCNC3=C(C=C(C=C3)[N+](=O)[O-])[N+](=O)[O-])C(=O)N[C@H](CCCNC(=N)N)C(=O)N)NC(=O)[C@@H]4CCCN4C(=O)[C@H](CCCCN)NC(=O)CNC(=O)C5=CC(=O)OC6=C5C=CC(=C6)OC.CC(=O)O

Cathepsin D and E FRET Substrate, acetate（Cat No.:I044279）is a synthetic peptide designed to measure the enzymatic activity of aspartic proteases cathepsin D and cathepsin E using fluorescence resonance energy transfer (FRET). The substrate contains a donor and acceptor fluorophore pair that emits fluorescence upon cleavage by the target enzymes. The acetate form refers to the peptide’s salt state, enhancing solubility and stability. This FRET substrate is widely used in biochemical assays, inhibitor screening, and protease kinetics studies, especially in cancer, neurodegeneration, and lysosomal function research where these enzymes are biologically relevant.