Carboxypeptidase B (MS grade) is a peptide exonuclease that can specifically degrade peptide chains. Carboxypeptidase B (MS grade) is progressively degraded from the C-terminal to release free amino acids. Carboxypeptidase B (MS grade) hydrolyzes only peptide bonds with basic amino acids (such as arginine and lysine) as C-terminal residues[1][2].
This product can be used for: 1) detection of antibody basic peaks; 2) determination of protein C-terminal amino acids.
Protocol
1) Dissolution: Dissolve carboxypeptidase B in pure water or 25mM Tris-HCl (pH 7.5-8.0) at a final concentration of 1-10mg/ml.
2) Enzyme digestion: The recommended ratio of carboxypeptidase B and target protein is 1:20-1:100 (w:w) for enzyme digestion, and the optimal enzyme digestion pH is 7.0-9.0.
| Catalog Number | I040142 |
| CAS Number | 9025-24-5 |
| Purity | ≥95% |
| Reference | [1]. FOLK JE, et al. The kinetics of carboxypeptidase B activity. II. Kinetic parameters of the cobalt and cadmium enzymes. J Biol Chem. 1962 Oct;237:3100-4. [2]. Qian X, et al. Molecular characterization of carboxypeptidase B-like (CPB) in Scylla paramamosain and its role in white spot syndrome virus and Vibrio alginolyticus infection. Fish Shellfish Immunol. 2019 Nov;94:434-446. |
