Bromophenol Blue

• CAT Number: M069289
• CAS Number: 115-39-9
• Molecular Formula: C19H10Br4O5S
• Molecular Weight: 669.96
• Purity: ≥95%
• Synonyms: 3,3-bis(3,5-dibromo-4-hydroxyphenyl)-3H-benzo[c][1,2]oxathiole 1,1-dioxide
• Target: Reagents
• Solubility: Soluble to 10 mM in ethanol
• Storage: Store at RT
• IUPAC Name: 2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-2,1$l^{6}
• InChI: InChI=1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
• InChIKey: UDSAIICHUKSCKT-UHFFFAOYSA-N
• SMILES: C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br

Bromophenol Blue (Cat No.: M069289) is a pH-sensitive dye and acid-base indicator commonly used in biochemical and molecular biology applications. It appears as a purple solid and exhibits a color change from yellow to blue over the pH range of 3.0 to 4.6. Widely employed as a tracking dye in gel electrophoresis, it helps monitor sample migration. Additionally, it serves as a staining reagent in protein assays and microscopy. Bromophenol Blue’s vivid color transition and solubility make it useful in analytical and diagnostic techniques.

Reference

1.Flores, R. "A rapid and reproducible assay for quantitative estimation of proteins using bromophenol blue." Analytical biochemistry 88.2 (1978): 605-611.
2.Ma, Chun Qi, Ke An Li, and Shen Yang Tong. "Microdetermination of proteins by resonance light scattering spectroscopy with bromophenol blue." Analytical biochemistry 239.1 (1996): 86-91.
3.BAKER, JOHN R. "Note on the use of bromophenol blue for the histochemical recognition of protein." Journal of Cell Science 3.48 (1958): 459-460.