Bromoacetamido-PEG2-C2-NHS ester

• CAT Number: I016454
• CAS Number: 1353011-78-5
• Molecular Formula: C13H19BrN2O7
• Molecular Weight: 395.20
• Purity: ≥95%
• Synonyms: (2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[(2-bromoacetyl)amino]ethoxy]ethoxy]propanoate
• InChI: InChI=1S/C13H19BrN2O7/c14-9-10(17)15-4-6-22-8-7-21-5-3-13(20)23-16-11(18)1-2-12(16)19/h1-9H2,(H,15,17)
• InChIKey: HDIXVXMKIWXBFA-UHFFFAOYSA-N
• SMILES: C1CC(=O)N(C1=O)OC(=O)CCOCCOCCNC(=O)CBr

Bromoacetamido-PEG2-C2-NHS ester(CAT: I016454) is a heterobifunctional crosslinker featuring an NHS ester for efficient coupling to primary amines on lysine side chains or protein N-termini, and a bromoacetamide group for selective thiol alkylation of cysteine residues. The PEG2-C2 spacer enhances hydrophilicity, reduces steric hindrance, and improves conjugate stability while maintaining flexibility for biomolecular interactions. This reagent is widely used in bioconjugation, antibody–drug conjugate (ADC) development, surface modification, and protein–protein interaction studies. By enabling site-specific and stable crosslinks, Bromoacetamido-PEG2-C2-NHS ester provides a versatile tool for constructing multifunctional biomolecules, biosensors, and drug delivery systems in chemical biology and materials research.