Brilliant Blue R250

• CAT Number: R017025
• CAS Number: 6104-59-2
• Molecular Formula: C45H44N3NaO7S2
• Molecular Weight: 825.97
• Purity: ≥95%
• Synonyms: sodium;3-[[4-[[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]cyclohexa-2,5-dien-1-ylidene]methyl]-N-ethylanilino]methyl]benzenesulfonate
• InChI: InChI=1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
• InChIKey: NKLPQNGYXWVELD-UHFFFAOYSA-M
• SMILES: CCN(CC1=CC(=CC=C1)S(=O)(=O)[O-])C2=CC=C(C=C2)C(=C3C=CC(=[N+](CC)CC4=CC(=CC=C4)S(=O)(=O)[O-])C=C3)C5=CC=C(C=C5)NC6=CC=C(C=C6)OCC.[Na+]

Brilliant Blue R250（Cat No.:R017025）is a commonly used Coomassie dye for staining proteins in polyacrylamide gels, particularly after SDS-PAGE. It binds non-specifically to proteins via electrostatic and hydrophobic interactions, producing an intense blue color that allows visualization of protein bands with high sensitivity and low background. Brilliant Blue R250 is favored for its rapid staining, ease of use, and compatibility with destaining procedures. It is widely employed in proteomics, biochemical research, and molecular biology for qualitative and semi-quantitative analysis of protein expression, purification, and characterization in gel electrophoresis workflows.