Biotin-PEG3-SS-azide

• CAT Number: I014203
• CAS Number: 2866429-93-6
• Molecular Formula: C27H48N8O7S3
• Molecular Weight: 692.91
• Purity: ≥95%
• Synonyms: 5-[(3aR,4R,6aS)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[3-[2-[[3-(3-azidopropylamino)-3-oxopropyl]disulfanyl]ethylamino]-3-oxopropoxy]ethoxy]ethoxy]ethyl]pentanamide
• IUPAC Name: 5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-N-[2-[2-[2-[3-[2-[[3-(3-azidopropylamino)-3-oxopropyl]disulfanyl]ethylamino]-3-oxopropoxy]ethoxy]ethoxy]ethyl]pentanamide
• InChI: InChI=1S/C27H48N8O7S3/c28-35-32-9-3-8-29-25(38)7-18-44-45-19-11-31-24(37)6-12-40-14-16-42-17-15-41-13-10-30-23(36)5-2-1-4-22-26-21(20-43-22)33-27(39)34-26/h21-22,26H,1-20H2,(H,29,38)(H,30,36)(H,31,37)(H2,33,34,39)/t21-,22-,26-/m0/s1
• InChIKey: FVMIKTRZXCSAEY-MCEYFSPLSA-N
• SMILES: C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCOCCOCCOCCC(=O)NCCSSCCC(=O)NCCCN=[N+]=[N-])NC(=O)N2

Biotin-PEG3-SS-azide（Cat No.:I014203）is a heterobifunctional crosslinker that integrates biotin, a PEG3 spacer, a cleavable disulfide bond, and an azide group. The biotin moiety enables strong, specific binding to streptavidin or avidin systems, while the azide group participates in bioorthogonal click chemistry with alkynes. The disulfide linker allows controlled cleavage under reducing conditions, providing reversible conjugation options. The PEG3 spacer imparts hydrophilicity, flexibility, and reduced steric hindrance, enhancing solubility and accessibility. This reagent is widely used in protein labeling, affinity purification, drug delivery, and biomolecular engineering applications.