Biotin-PEG12-NHS ester

• CAT Number: I015738
• CAS Number: 1934296-88-4
• Molecular Formula: C41H72N4O18S
• Molecular Weight: 941.09
• Purity: ≥95%
• Synonyms: (2,5-dioxopyrrolidin-1-yl) 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate
• InChI: InChI=1S/C41H72N4O18S/c46-36(4-2-1-3-35-40-34(33-64-35)43-41(50)44-40)42-8-10-52-12-14-54-16-18-56-20-22-58-24-26-60-28-30-62-32-31-61-29-27-59-25-23-57-21-19-55-17-15-53-13-11-51-9-7-39(49)63-45-37(47)5-6-38(45)48/h34-35,40H,1-33H2,(H,42,46)(H2,43,44,50)/t34-,35-,40-/m0/s1
• InChIKey: WOLXSUFTTIRQOU-BVCQTOFBSA-N
• SMILES: C1CC(=O)N(C1=O)OC(=O)CCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCNC(=O)CCCC[C@H]2[C@@H]3[C@H](CS2)NC(=O)N3

Biotin-PEG12-NHS ester（Cat No.:I015738）is a long, hydrophilic biotinylation reagent designed for efficient and stable conjugation. It contains a biotin group for strong streptavidin/avidin binding, a PEG12 spacer that enhances solubility, reduces steric hindrance, and increases flexibility, and an NHS ester that reacts selectively with primary amines to form stable amide bonds. The extended PEG12 chain improves accessibility and minimizes aggregation, making it highly suitable for use in complex biological environments. This reagent is widely applied in protein labeling, imaging, affinity purification, and bioconjugation research.