Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA

• CAT Number: I045455
• Molecular Formula: C38H66F3N13O11S
• Molecular Weight: 970.07
• Purity: ≥95%
• Synonyms: N-[3-[2-[2-[3-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]propoxy]ethoxy]ethoxy]propyl]-6-[[2-[[(2S)-2-[(2-azidoacetyl)amino]-5-(diaminomethylideneamino)pentanoyl]amino]acetyl]amino]hexanamide;2,2,2-trifluoroacetic acid
• InChI: InChI=1S/C36H65N13O9S.C2HF3O2/c37-35(38)43-14-6-9-26(46-32(53)24-45-49-39)34(54)44-23-31(52)42-13-5-1-2-11-29(50)40-15-7-17-56-19-21-58-22-20-57-18-8-16-41-30(51)12-4-3-10-28-33-27(25-59-28)47-36(55)48-33;3-2(4,5)1(6)7/h26-28,33H,1-25H2,(H,40,50)(H,41,51)(H,42,52)(H,44,54)(H,46,53)(H4,37,38,43)(H2,47,48,55);(H,6,7)/t26-,27-,28-,33-;/m0./s1
• InChIKey: KAWIHMNFOPBGAK-LXKMWQMESA-N
• SMILES: C1C2C(C(S1)CCCCC(=O)NCCCOCCOCCOCCCNC(=O)CCCCCNC(=O)CNC(=O)C(CCCN=C(N)N)NC(=O)CN=[N+]=[N-])NC(=O)N2.C(=O)(C(F)(F)F)O

Biotin-C1-PEG3-C3-amido-C5-Gly-Arg-Gly-N3 TFA (Cat No.: I045455) is a multifunctional bioconjugation reagent composed of a biotin moiety for high-affinity streptavidin/avidin binding, a PEG3 spacer for solubility and flexibility, and a Gly-Arg-Gly tripeptide for biomolecular compatibility. It features an azide (N3) group for click chemistry coupling and a trifluoroacetic acid (TFA) salt form for stability. The C1, C3, and C5 spacers provide optimal separation between functional domains, reducing steric hindrance. This reagent is widely used in chemical biology, proteomics, and targeted delivery applications for selective labeling and conjugation.

Reference

[1]. Malgorzata Broncel, et al. Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic profiling of protein lipidation during vertebrate development. Angew Chem Int Ed Engl. 2015 May 11;54(20):5948-51.
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