BCN-PEG3-Biotin

• CAT Number: I045960
• CAS Number: 1263166-92-2
• Molecular Formula: C29H46N4O7S
• Molecular Weight: 594.76
• Purity: ≥95%
• Synonyms: [(1S,8R)-9-bicyclo[6.1.0]non-4-ynyl]methyl N-[2-[2-[2-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethoxy]ethoxy]ethoxy]ethyl]carbamate
• InChI: InChI=1S/C29H46N4O7S/c34-26(10-6-5-9-25-27-24(20-41-25)32-28(35)33-27)30-11-13-37-15-17-39-18-16-38-14-12-31-29(36)40-19-23-21-7-3-1-2-4-8-22(21)23/h21-25,27H,3-20H2,(H,30,34)(H,31,36)(H2,32,33,35)/t21-,22+,23?,24-,25-,27-/m0/s1
• InChIKey: LZIHJLCCQAFQSO-MSBMMZNKSA-N
• SMILES: C1CC2C(C2COC(=O)NCCOCCOCCOCCNC(=O)CCCCC3C4C(CS3)NC(=O)N4)CCC#C1

BCN-PEG3-Biotin(CAT: I045960) is a heterobifunctional bioconjugation reagent consisting of a bicyclo[6.1.0]nonyne (BCN) group, a short PEG3 spacer, and a biotin moiety. The BCN group enables fast, copper-free strain-promoted azide–alkyne cycloaddition (SPAAC) with azide-functionalized molecules, making it ideal for bioorthogonal labeling under mild conditions. The PEG3 linker enhances solubility and flexibility while reducing steric hindrance between the reactive BCN and biotin. The biotin group provides strong, specific binding to streptavidin and avidin, enabling sensitive detection, purification, and immobilization applications. This reagent is widely used in chemical biology, diagnostics, and drug delivery research for site-specific and efficient bioconjugation.