Azido-PEG3-Val-Cit-PAB-PNP

• CAT Number: I016807
• CAS Number: 2055047-18-0
• Molecular Formula: C34H47N9O12
• Molecular Weight: 773.79
• Purity: ≥95%
• Synonyms: [4-[[(2S)-2-[[(2S)-2-[3-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]propanoylamino]-3-methylbutanoyl]amino]-5-(carbamoylamino)pentanoyl]amino]phenyl]methyl (4-nitrophenyl) carbonate
• InChI: InChI=1S/C34H47N9O12/c1-23(2)30(41-29(44)13-16-51-18-20-53-21-19-52-17-15-38-42-36)32(46)40-28(4-3-14-37-33(35)47)31(45)39-25-7-5-24(6-8-25)22-54-34(48)55-27-11-9-26(10-12-27)43(49)50/h5-12,23,28,30H,3-4,13-22H2,1-2H3,(H,39,45)(H,40,46)(H,41,44)(H3,35,37,47)/t28-,30-/m0/s1
• InChIKey: HLBCDJANEWKOSX-JDXGNMNLSA-N
• SMILES: CC(C)C(C(=O)NC(CCCNC(=O)N)C(=O)NC1=CC=C(C=C1)COC(=O)OC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)CCOCCOCCOCCN=[N+]=[N-]

Azido-PEG3-Val-Cit-PAB-PNP(CAT: I016807) is a cleavable, heterobifunctional linker widely applied in antibody–drug conjugate (ADC) development and targeted drug delivery. It features an azide group for copper-catalyzed azide–alkyne cycloaddition (“click chemistry”) and a para-nitrophenyl (PNP) ester that reacts efficiently with amines, enabling site-specific conjugation. The Val-Cit-PAB sequence is a cathepsin B–cleavable dipeptide linker that undergoes enzymatic degradation in lysosomes, ensuring controlled payload release. The PEG3 spacer improves solubility, reduces steric hindrance, and provides flexibility. Azido-PEG3-Val-Cit-PAB-PNP is a valuable tool in bioconjugation, enabling the construction of stable, cleavable linkers for ADCs and other targeted therapeutic strategies.