Amine-PEG4-Desthiobiotin

• CAT Number: I044699
• CAS Number: 2306109-91-9
• Molecular Formula: C20H40N4O6
• Molecular Weight: 432.55
• Purity: ≥95%
• Synonyms: N-[2-[2-[2-[2-(2-aminoethoxy)ethoxy]ethoxy]ethoxy]ethyl]-6-[(4R,5S)-5-methyl-2-oxoimidazolidin-4-yl]hexanamide
• InChI: InChI=1S/C20H40N4O6/c1-17-18(24-20(26)23-17)5-3-2-4-6-19(25)22-8-10-28-12-14-30-16-15-29-13-11-27-9-7-21/h17-18H,2-16,21H2,1H3,(H,22,25)(H2,23,24,26)/t17-,18+/m0/s1
• InChIKey: DQZZCBCJTNOZDL-ZWKOTPCHSA-N
• SMILES: C[C@H]1[C@H](NC(=O)N1)CCCCCC(=O)NCCOCCOCCOCCOCCN

Amine-PEG4-Desthiobiotin（Cat No.:I044699）is a bioconjugation reagent that combines a terminal primary amine group, a PEG4 (tetraethylene glycol) spacer, and desthiobiotin, a biotin analog with reversible binding to streptavidin. The amine allows covalent attachment to activated esters (e.g., NHS-esters) on biomolecules, while the PEG4 spacer enhances water solubility and reduces steric hindrance. Desthiobiotin provides strong, but reversible, binding to streptavidin, allowing for gentle elution of captured molecules in affinity purification. This reagent is widely used in proteomics, pull-down assays, and bioorthogonal labeling where reversible biotin-streptavidin interactions are advantageous.