AFMK

• CAT Number: R001284
• CAS Number: 52450-38-1
• Molecular Formula: C13H16N2O4
• Molecular Weight: 264.28
• Purity: ≥95%
• Synonyms: N-[3-(2-formamido-5-methoxyphenyl)-3-oxopropyl]acetamide
• InChI: InChI=1S/C13H16N2O4/c1-9(17)14-6-5-13(18)11-7-10(19-2)3-4-12(11)15-8-16/h3-4,7-8H,5-6H2,1-2H3,(H,14,17)(H,15,16)
• InChIKey: JYWNYMJKURVPFH-UHFFFAOYSA-N
• SMILES: CC(=O)NCCC(=O)C1=C(C=CC(=C1)OC)NC=O

AFMK (Cat No.: R001284) is a major metabolite of melatonin produced via enzymatic and oxidative pathways. It functions as a potent free radical scavenger and antioxidant, protecting cells from oxidative and nitrosative stress. AFMK also exhibits anti-inflammatory and neuroprotective properties, contributing to melatonin’s broader physiological effects. It has been studied in contexts such as neurodegeneration, immune regulation, and mitochondrial function. In research, AFMK is widely used as a biomarker and probe to explore melatonin metabolism and indoleamine-related signaling pathways.

Reference

[1]. Annia Galano, et al. On the free radical scavenging activities of melatonin’s metabolites, AFMK and AMK. J Pineal Res. 2013 Apr;54(3):245-57.
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[2]. Kailash Manda, et al. AFMK, a melatonin metabolite, attenuates X-ray-induced oxidative damage to DNA, proteins and lipids in mice. J Pineal Res. 2007 Apr;42(4):386-93.
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[3]. Anna Leja-Szpak, et al. Melatonin and its metabolite N1-acetyl-N2-formyl-5-methoxykynuramine (afmk) enhance chemosensitivity to gemcitabine in pancreatic carcinoma cells (PANC-1). Pharmacol Rep. 2018 Dec;70(6):1079-1088.
[Content Brief]