AF647-NHS ester triTEA

• CAT Number: I040436
• CAS Number: 407627-61-6
• Molecular Formula: C58H94N6O16S4
• Molecular Weight: 1259.66
• Purity: ≥95%
• Synonyms: N,N-diethylethanamine;3-[(2Z)-2-[(2E,4E)-5-[3,3-dimethyl-5-sulfo-1-(3-sulfopropyl)indol-1-ium-2-yl]penta-2,4-dienylidene]-3-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3-methyl-5-sulfoindol-1-yl]propane-1-sulfonate
• InChI: InChI=1S/C40H49N3O16S4.3C6H15N/c1-39(2)30-26-28(62(53,54)55)15-17-32(30)41(22-10-24-60(47,48)49)34(39)12-6-4-7-13-35-40(3,21-9-5-8-14-38(46)59-43-36(44)19-20-37(43)45)31-27-29(63(56,57)58)16-18-33(31)42(35)23-11-25-61(50,51)52;3*1-4-7(5-2)6-3/h4,6-7,12-13,15-18,26-27H,5,8-11,14,19-25H2,1-3H3,(H3-,47,48,49,50,51,52,53,54,55,56,57,58);3*4-6H2,1-3H3
• InChIKey: ZSJDPEXOCKLWTC-UHFFFAOYSA-N
• SMILES: CCN(CC)CC.CCN(CC)CC.CCN(CC)CC.CC1(C2=C(C=CC(=C2)S(=O)(=O)O)[N+](=C1C=CC=CC=C3C(C4=C(N3CCCS(=O)(=O)[O-])C=CC(=C4)S(=O)(=O)O)(C)CCCCCC(=O)ON5C(=O)CCC5=O)CCCS(=O)(=O)O)C

AF647-NHS ester(CAT: I040436) is a reactive fluorescent dye analogue of Alexa Fluor 647 (AF647), designed for efficient labeling of biomolecules containing primary amines, such as proteins, antibodies, and peptides. Featuring an N-hydroxysuccinimide (NHS) ester group, it enables stable amide bond formation under mild aqueous conditions. AF647-NHS ester emits in the far-red/near-infrared spectrum, providing bright fluorescence, excellent photostability, and low background interference, ideal for high-resolution imaging and multiplexed detection. It is widely used in fluorescence microscopy, flow cytometry, and Western blotting applications. Its superior solubility and stability make it a reliable tool for advanced bioimaging and molecular tagging in complex biological systems.