For research use only. Not for therapeutic Use.
Ac-Ile-Glu-Thr-Asp-pNA（Cat No.:I045781）is a synthetic tetrapeptide substrate commonly used in enzymatic assays to study caspase activity, particularly caspase-8. The sequence Ile-Glu-Thr-Asp (IETD) is recognized and cleaved at the aspartic acid residue, releasing para-nitroaniline (pNA), which produces a measurable colorimetric signal at 405 nm. This enables sensitive and quantitative monitoring of protease activity in apoptosis research. With its N-terminal acetylation for stability, Ac-IETD-pNA serves as a reliable biochemical tool for evaluating caspase inhibitors, screening drug candidates, and exploring programmed cell death mechanisms in disease models.
| CAS Number | 219138-21-3 |
| Synonyms | (4S)-4-[[(2S,3S)-2-acetamido-3-methylpentanoyl]amino]-5-[[(2S,3R)-1-[[(2S)-3-carboxy-1-(4-nitroanilino)-1-oxopropan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-5-oxopentanoic acid |
| Molecular Formula | C27H38N6O12 |
| Purity | ≥95% |
| IUPAC Name | (4S)-4-[[(2S,3S)-2-acetamido-3-methylpentanoyl]amino]-5-[[(2S,3R)-1-[[(2S)-3-carboxy-1-(4-nitroanilino)-1-oxopropan-2-yl]amino]-3-hydroxy-1-oxobutan-2-yl]amino]-5-oxopentanoic acid |
| InChI | InChI=1S/C27H38N6O12/c1-5-13(2)22(28-15(4)35)26(42)30-18(10-11-20(36)37)24(40)32-23(14(3)34)27(43)31-19(12-21(38)39)25(41)29-16-6-8-17(9-7-16)33(44)45/h6-9,13-14,18-19,22-23,34H,5,10-12H2,1-4H3,(H,28,35)(H,29,41)(H,30,42)(H,31,43)(H,32,40)(H,36,37)(H,38,39)/t13-,14+,18-,19-,22-,23-/m0/s1 |
| InChIKey | FHURKTIGZAIQGR-BQCLNCLCSA-N |
| SMILES | CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)C |
| Chemistry Calculators | Dilution Calculator In vivo Formulation Calculator Molarity Calculator Molecular Weight Calculator Reconstitution Calculator |
