5'-ODMT cEt N-Bzm5 C Phosphoramidite (Amidite)

• CAT Number: I042307
• CAS Number: 1197033-17-2
• Molecular Formula: C49H56N5O9P
• Molecular Weight: 889.97
• Purity: ≥95%
• Synonyms: N-[1-[(1S,3R,4R,6S,7S)-1-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-7-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxy-6-methyl-2,5-dioxabicyclo[2.2.1]heptan-3-yl]-5-methyl-2-oxopyrimidin-4-yl]benzamide
• InChI: InChI=1S/C49H56N5O9P/c1-32(2)54(33(3)4)64(60-29-15-28-50)63-43-42-46(53-30-34(5)44(52-47(53)56)51-45(55)36-16-11-9-12-17-36)62-48(43,35(6)61-42)31-59-49(37-18-13-10-14-19-37,38-20-24-40(57-7)25-21-38)39-22-26-41(58-8)27-23-39/h9-14,16-27,30,32-33,35,42-43,46H,15,29,31H2,1-8H3,(H,51,52,55,56)/t35-,42+,43-,46+,48-,64?/m0/s1
• InChIKey: BDTXILPCBUAYPJ-USFFSJIASA-N
• SMILES: CC1C2(C(C(O1)C(O2)N3C=C(C(=NC3=O)NC(=O)C4=CC=CC=C4)C)OP(N(C(C)C)C(C)C)OCCC#N)COC(C5=CC=CC=C5)(C6=CC=C(C=C6)OC)C7=CC=C(C=C7)OC

5′-ODMT cEt N-Bzm5 C Phosphoramidite (Amidite) (Cat No.: I042307) is a chemically modified cytidine phosphoramidite used in oligonucleotide synthesis. It features a 5′-O-dimethoxytrityl (ODMT) protecting group, a constrained ethyl (cEt) modification for enhanced affinity and stability, and an N4-benzoyl (N-Bzm5) protective group on the cytosine base. This amidite enhances the pharmacokinetic and biophysical properties of oligonucleotides, improving nuclease resistance and hybridization strength. It is widely utilized in the development of antisense oligonucleotides, siRNA, and other nucleic acid-based therapeutics and research tools.

Reference

[1]. Yu J, et al. Synthesis and antisense properties of 2′-O-(2S-methoxypropyl)-RNA-modified gapmer antisense oligonucleotides. ChemMedChem. 2014 Sep;9(9):2040-4.
[Content Brief]