5-Me-dC(Ac) amidite

• CAT Number: I042979
• CAS Number: 1873306-74-1
• Molecular Formula: C42H52N5O8P
• Molecular Weight: 785.86
• Purity: ≥95%
• Synonyms: N-[1-[(2R,5R)-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyoxolan-2-yl]-5-methyl-2-oxopyrimidin-4-yl]acetamide
• IUPAC Name: N-[1-[(2R,4S,5R)-5-[[bis(4-methoxyphenyl)-phenylmethoxy]methyl]-4-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxyoxolan-2-yl]-5-methyl-2-oxopyrimidin-4-yl]acetamide
• InChI: InChI=1S/C42H52N5O8P/c1-28(2)47(29(3)4)56(53-24-12-23-43)55-37-25-39(46-26-30(5)40(44-31(6)48)45-41(46)49)54-38(37)27-52-42(32-13-10-9-11-14-32,33-15-19-35(50-7)20-16-33)34-17-21-36(51-8)22-18-34/h9-11,13-22,26,28-29,37-39H,12,24-25,27H2,1-8H3,(H,44,45,48,49)/t37-,38+,39+,56?/m0/s1
• InChIKey: YFUQEQDYBKCNTE-CMFYRTQUSA-N
• SMILES: CC1=CN(C(=O)N=C1NC(=O)C)[C@H]2C[C@@H]([C@H](O2)COC(C3=CC=CC=C3)(C4=CC=C(C=C4)OC)C5=CC=C(C=C5)OC)OP(N(C(C)C)C(C)C)OCCC#N

5-Me-dC(Ac) amidite（Cat No.:I042979）is a modified nucleotide amidite used in the synthesis of oligonucleotides. It contains a 5-methyl group attached to the cytosine base, which mimics the naturally occurring methylation pattern in DNA, and an acetyl group (Ac) on the nucleoside’s amine group. This modification is commonly employed in research to study DNA methylation and gene regulation, as the 5-methylcytosine plays a critical role in epigenetic processes. The acetyl group enhances the stability and reactivity of the amidite, making it useful in solid-phase DNA synthesis for various molecular biology applications.