5-Carboxytetramethylrhodamine

• CAT Number: M100672
• CAS Number: 150322-05-7
• Molecular Formula: C25H22N2O5
• Molecular Weight: 430.45
• Purity: ≥95%
• Synonyms: 3′,6′-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylic acid
• IUPAC Name: 3',6'-bis(dimethylamino)-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• InChI: InChI=1S/C25H22N2O5/c1-26(2)15-6-9-19-21(12-15)31-22-13-16(27(3)4)7-10-20(22)25(19)18-8-5-14(23(28)29)11-17(18)24(30)32-25/h5-13H,1-4H3,(H,28,29)
• InChIKey: DCVXPZDNLDPUES-UHFFFAOYSA-N
• SMILES: CN(C)C1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)C(=O)O)C(=O)O3)C5=C(O2)C=C(C=C5)N(C)C

5-Carboxytetramethylrhodamine (Cat No.:M100672) is a bright, red-orange fluorescent dye commonly used for labeling biomolecules in fluorescence-based assays. Featuring a carboxyl group at the 5-position, it allows for efficient conjugation to amine-containing compounds via amide bond formation. 5-TAMRA exhibits high photostability, strong absorption (~546 nm), and intense emission (~579 nm), making it suitable for applications such as fluorescence microscopy, flow cytometry, and FRET-based detection. Its well-defined spectral properties and compatibility with various labeling strategies make 5-TAMRA a valuable tool in molecular biology, diagnostics, and bioimaging research.