Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) General Information
Product name
Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12)
Validated applications
ELISA
Specificity
Specifically recognizes MMAF and does not recognize MMAE.
Immunogen
MMAF-BSA
Source
Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
Purification
Protein A purified / Protein G purified
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. Contact us for customized product form or formulation.
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Recommended Usage
0.06-125 ng/mL
Note
MuseChem can customize this product per customer's request including product size, buffer components, etc.
Clone ID
1M1E12
Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) Images
Immobilized IgG1-MMAF at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. AB00010) with a linear range of 0.06-2 ng/mL (QC tested).
Serial dilutions of MMAF were added into Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. AB00010): IgG1-MMAF binding reactions. The half maximal inhibitory concentration (IC50) is 0.1106 μg/mL (Routinely tested).
ELISA binding of Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. AB00010) with Doxorubicin-ADC, Disitamab Vedotin (RC48), IgG1-MMAF, Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 conju-gated antibody respectively.
The coating antibody was Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. AB00010), used at 1 μg/mL concentration. The primary an-tibody were different payload conjugated antibodies, including Doxorubicin-ADC, Disitamab Vedotin (RC48), IgG1-MMAF, Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 conjugated antibodies used at 0.25 μg/mL con-centration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Anti-body (6F11C8), mAb used at 1:10000 concentration.
Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. AB00010) is specific to IgG1-MMAF and has no cross-reactivity with Doxorubi-cin-ADC, Disitamab Vedotin (RC48), Trastuzumab Deruxtecan, Sacituzumab Govitecam and Trastuzumab-DM1 (Routinely tested).
